I didn't experienced with Assist, but I did with MCS.
First, please check 'Transfer' setting in Assist. In case of MCS, 'Transfer' setting is needed.
Second, 'telestreamplayback' of 'tm:5534/telestreamplayback' is not the name of target FTP server.
That is the profile name containing setting of FTP server.
If you don't have success in spite of trying above, you can try removeing port number from 'tm:5534/telestreamplayback' .
If you can't solve your TM problem yet, I hope my experience is helpful to you.
Can you send from an edit client successfully?
For Assist, you'll need to look into your Transfer/Media Services settings in Interplay. Also, check out the permissions on your Assist users. Sending to playback is something that can be enabled and disabled in Interplay User management.
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